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Target gene primer sequences.
| Primer | Primer sequence (5′-3′) | Restriction sites |
|---|---|---|
| UOX-F | GGTCGACATGTCAACAACGCTCTCATCCACC | SalI |
| UOX-R | GCGAAGCTTTTAGTGGTGGTGGTGGTGGTGCAACTTG | HindIII |
| sUOX-F | CGTCTAGAATGACTTTACAAATACATACAGGGGGTATTAATTTGAAAAAG | XbaI |
| sUOX-R | ATAAAGCTTTTAGTGGTGGTGGTGGTGGTG | HindIII |
The underlined letters indicate restriction enzyme cutting sites. UOX, uricase; sUOX, Staphylococcal Protein A + uricase; F, forward; R, reverse.
