Figure 3.

Mel reduces oxidative stress in OGD/R-exposed H9c2 cells. H9c2 cells were subjected to 4 h of OGD followed by reperfusion for 24 h. Mel (1 mM) was added to the culture medium at the initiation of reperfusion. (A) ROS production was detected using a 2′,7′-dichlorofluorescein diacetate fluorescent probe. (B) MDA content and the activity of (C) SOD, (D) CAT and (E) GSH-Px were measured using commercial kits. Data are expressed as the mean ± SD from three independent experiments. ^*P<0.05 vs. control; ^#P<0.05 vs. OGD/R. Mel, melatonin; OGD/R, oxygen-glucose deprivation/reperfusion; ROS, reactive oxygen species; MDA, malondialdehyde; SOD, superoxide dismutase; CAT, catalase; GSH-Px, glutathione peroxidase.