Table II.
Primers used in the present study.
| Gene | Forward primer sequence (5′-3′) | Reverse primer sequence (5′-3′) |
|---|---|---|
| hilA | ATTAAGGCGACAGAGCTGGA | ATAGCAAACTCCGACGATG |
| invF | TGTCGCACCAGTATCAGGAG | AAATAGCGCGAAACTCAGGA |
| ssaG | GATATGCTCTCCACATGGC | AGGCAAATTGCGCTTTAATC |
| ssaB | GGATTCATGCTGGCAGTTTT | GCAGGATGCCATCAATAGT |
| sseA | AGGAGGCCGAGAAGGATTTA | TTCCTGACGGTATCTCCACC |
| ssrA | CATGTTTGTGGCACTATCCG | GCGAGCTAAGGTAGCCAATG |
| sipA | AAACGTTGATACCTGCTG | GACATGGCTTCGGCTATTGT |
| sipB | TGTTTCGCTGTCTCAACTGG | TCGCAGCGTCATAAACACTC |
| sipC | AAGTCAGTGACCTGGGGTTG | AACGGCACTGGAAGACATTC |
| sipD | ACTGCCGTGGTACCGTCTAC | ATAATGACACGCCGGACTTC |
| fthD | CAACGAAGAGATGGCAAACA | GACGCGTTGAAAGCATGATA |
| fliA | CGCTGAAGGTGTAATGGAT | CCGCATTTAATAACCGATG |
| fliZ | AAACATTTCCACGATCTGC | CGGTAAAGGGGGATTTCTGT |
| flgM | CTTTGAAACCGTTAGCAC | GCCGTTTTTAATGCTTCGAC |
| fliC | AACGACGGTATCTCCATTGC | TACACGGTCGATTTCGTTCA |
| pts Imutant | AAGTTTTTTTTCCGGGTTCTTTTAAAAATCA | GCAGTTCCTGTTTGTAGATTTCTTTGCGCAG |
| Construction | GTCACAAGTAAGGTAGGGTTCATATGAATATCCTCCTTA | CGCGCGAACTTCTTGTCAATCTAGGCTGGAGCTGCTTC |