Figure 6.

SB inhibits RCC EMT and metastasis in vivo. (A) 786-O cells were subcutaneously injected into male nude mice. Tumors were isolated from male BALB/c mice and measured. (B) Tumor volumes were measured every three days in the DMSO or SB treated mice. (C) After 30 days, the mice were sacrificed and tumor weights in mice injected with 786-O cells. Data are presented as the mean ± standard deviation. ^*P<0.05 and ^**P<0.01. (D) Western blot analysis of β-catenin, E-cadherin, vimentin and LC3-I/II expression in tumor xenografts. (E) Immunohistochemical analysis of β-catenin, E-cadherin and vimentin in tumor xenografts. (F) Luciferase-tagged 786-O cells were intravenously injected into male nude mice through the tail vein. Representative bioluminescence images of mice treated with DMSO or SB. (n=5). RCC, renal cell carcinoma; EMT, epithelial-mesenchymal transition; SB, silibinin; N, neural; E, epithelial.