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. 2020 Mar 20;14(3):e0008126. doi: 10.1371/journal.pntd.0008126

Fig 10. Migration of prostate cancer cells is induced by conditioned medium of M2-like macrophages cultured with TCM.

Fig 10

Prostate cancer cells (PC3, DU145 and LNCaP) were cultured with CM, TCM, M-CM, M-TCM or αgp130+M-TCM for 24 hr. (A) Migration of the prostate cancer cells was assessed by wound healing assays. Representative images photographed by light microscope. (B) Migration was measured as: {(mean wounded breadth–mean remaining breadth)} / mean wounded breadth × 100. Data are means ± SD of three independent experiments. *p<0.05 versus untreated prostate cancer cells (C). #p<0.05 versus conditioned medium of RWPE-1 stimulated with T. vaginalis (TCM). CM: conditioned medium of RWPE-1 alone, TCM: conditioned medium of RWPE-1 stimulated with T. vaginalis, M-CM: conditioned medium of THP-1-derived macrophages stimulated with CM, M-TCM: conditioned medium of THP-1-derived macrophages stimulated with TCM. αgp130+M-TCM: conditioned medium of THP-1-derived macrophage stimulated with TCM after pretreatment of gp130 antibody.