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. 2020 Jan 29;14(4):763–778. doi: 10.1002/1878-0261.12641

Figure 2.

Figure 2

NEK2 modulates autophagy in MM cells. (A) Western blots of NEK2, LC3B, and β‐actin in KMS11 EV, KMS11 NEK2‐OE, RPMI 8226 EV, and RPMI 8226 NEK2‐OE MM cells. (B) Western blots of NEK2, LC3B, and β‐actin in KMS11 Scr, KMS11 NEK2‐shRNA, RPMI 8226 Scr, and RPMI 8226 NEK2‐shRNA MM cells. (C) KMS11 EV, KMS11 NEK2‐OE, RPMI 8226 EV, and RPMI 8226 NEK2‐OE MM cells imaging with 0.5 μm DALGreen staining. (D) KMS11 Scr, KMS11 NEK2‐shRNA, RPMI 8226 Scr, and RPMI 8226 NEK2‐shRNA imaging with 0.5 μm DALGreen staining. (E) TEM analysis of autophagosomes in KMS11 EV and KMS11 NEK2‐OE MM cells. (F) TEM analysis of autophagosomes in KMS11 Sr and KMS11 NEK2‐shRNA MM cells. Scale bar: 5 or 2 μm. **P < 0.01, ****P < 0.0001. Significance was determined by Student’s t‐test. Error bars indicate SD.