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. 2020 Feb 13;14(4):779–794. doi: 10.1002/1878-0261.12640

Figure 5.

Figure 5

CCL5 overexpression in MOLM‐13 sensitive cells leads to sustained resistance to PKC412 (A) MOLM‐13‐sensitive cells were transiently transfected with a CCL5‐overexpressing vector or an empty control vector. Expression on protein level was higher 72 h after transfection. (B) TKI‐sensitive MOLM‐13 cells were transiently transfected with a CCL5 expressing vector or the corresponding empty control vector and were replated in serum‐free medium 48 h after transfection followed by treatment with PKC412 at the indicated concentrations. Proliferation was measured using MTS assay 48 h after addition of PKC412. (C) and (D) TKI‐sensitive MOLM‐13 cells were transiently transfected with a CCL5‐expressing vector or an empty control vector and were replated into serum‐free medium 48‐h after transfection followed by treatment with 50 and 100 nm PKC412. After 12 h, cells were analyzed via intracellular flow cytometric analysis for the levels of (C) p‐Akt and (D) p‐Stat5. Significant differences are marked with (*): *P < 0.05, **P < 0.01, ***P < 0.001. Student’s t‐test. Error bars represent SD. N = 3 for (A–D).