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. 2020 Feb 28;14(4):865–881. doi: 10.1002/1878-0261.12643

Figure 5.

Figure 5

This protocol enables to capture PD‐L1+CTCs that express GC ‐specific marker, HER‐2. Immunofluorescent staining of CSV (84‐1, green), CD45 (red), PD‐L1 (red), EpCAM (green), and HER‐2 (red) in CTCs from an HER‐2‐positive GC patient’s blood sample captured by CSV (A) and EpCAM (B). Scale bar, 10 μm. (C) Representative images for HER‐2 immunohistochemical staining image from the same case. The original magnification is 20 × 10. NC, negative control, means a staining without adding the primary antibody.