Figure 3.

Ectopic expression of BCAM promotes GC cell migration and invasion. (A) Immunoblot analysis of BCAM expression levels following the treatment of SGC‐7901 cells with an empty vector and pCS2‐BCAM. (B–D) SGC‐7901 cells transfected with the indicated plasmids were processed for MTT assay (B), colony‐forming assay (C), and cell cycle analysis (D). The bar chart for cell cycle represents the percentage of cells in the G0/G1, S, or G2/M phase, as indicated. (E, F) Transwell migration (E) and Matrigel invasion assays (F) were carried out using GC cells with the indicated treatments. Migrated and invaded cells were counted. Scar bar, 100 μm. Experiments were performed in triplicate. Data are presented as the means ± SDs; ns, no significance; **P < 0.01.