Skip to main content
. 2020 Feb 11;12(6):4727–4741. doi: 10.18632/aging.102796

Figure 6.

Figure 6

Working mechanism of metformin in the INFH rat model. (A) Immunohistochemical staining of ALPL, DMP1, vWF, and TRAP in the femoral head epiphysis. Black arrows indicate positive expression of vWF, a marker of endothelial cells of blood vessels. (B) Percentage of vascular density in the four groups. Vascular density (%) was calculated as vascular area stained by antibody for vWF-related antigen/total area of each image. Values are presented as the mean ± STD. (n = 4). *p < 0.01 versus Sham Control, #p < 0.01 versus INFH Control. (C) Number of TRAP-positive cells per unit area. Values are presented as the mean ± STD (n = 4). **p < 0.001 versus Sham Control, ##p < 0.001 versus INFH Control. Black bars = 50 μm.