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. 2020 Mar 19;12(6):5091–5120. doi: 10.18632/aging.102933

Figure 5.

Figure 5

Activation of circulating platelets in mice injected with 4T1 cancer cells or saline. Results are presented as median (horizontal line) and interquartile range (box) (n = 8). The expressions of P-selectin (CD62P) (A), the active form of GPIIb/IIIa (B) and the binding of endogenous vWF (C) and endogenous fibrinogen (Fg) (D) on resting platelets were measured using flow cytometry in non-fixed ‘washed blood’ withdrawn immediately (t0) or after 5 weeks (t5) from the injection of mice with 4T1 cancer cells or saline. Results are expressed as the percent fraction of platelets positive for a given activation marker. More experimental details are given in the Materials and methods section. The statistical significance of differences, estimated with Kruskal-Wallis test followed by the post hoc Conover-Inman all-pairwise comparisons test, P-selectinresting, P1,α < 0.001, 4T1 t5 > saline t5; P1,α < 0.001, 4T1 t5 > 4T1 t0; active form of GPIIb/IIIaresting, P1,α < 0.001, 4T1 t5 > saline t5; P1,α < 0.001, 4T1 t5 > 4T1 t0; vWFresting, P1,α < 0.001, 4T1 t5 > saline t5; P1,α < 0.001, 4T1 t5 > 4T1 t0; Fgresting, P1,α < 0.001, 4T1 t5 > saline t5; P1,α < 0.001, 4T1 t5 > 4T1 t0.