Skip to main content
. 2020 Mar 25;12(6):5362–5383. doi: 10.18632/aging.102956

Figure 5.

Figure 5

Delphinidin downregulated NOX through activating AMPK. (A) Determination of rac1 activity. Cell lysates were affinity precipitated with GTP-PBD bound to glutathione-agarose beads. Precipitated GTP-Rac1 was detected by immunoblotting with anti-Rac1 antibody (n=5). ***p<0.001 versus the PBS group; #p<0.05 versus the Ang II group. (B) Expression of the NOX subunits p47phox, p40phox, p67phox, gp91phox, p22phox (n=5). *p<0.05 versus the PBS group; ns versus the Ang II group; #p<0.05 versus the Ang II group. (C) Representative western blot analysis revealed AMPK phosphorylation levels (n=5). **p<0.01 versus the PBS group; ##p<0.01 versus the Ang II group. (D) Representative western blot analysis and GST pulldown analysis revealed the effect of delphinidin on the AMPK phosphorylation level and NADPH oxidase subunit p47phox and Rac1 activity. **p<0.01 versus the PBS group; #p<0.05 versus the Ang II group; ##p<0.01 versus the Ang II group; $p<0.05 versus the Ang II+Dp group; $$p<0.01 versus the Ang II+Dp group. Bubbles and traces besides the main strips are parts of the blotting background in western blot.