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. 2020 Apr 7;9:e53582. doi: 10.7554/eLife.53582

Figure 1. Generation of Tg-Cyp4f39–/– mice.

Figure 1.

(A) Schematic illustration of the cornea, tear film, and structures of major meibum lipids (wax ester [WE], cholesteryl ester [CE], and (O-Acyl)-ω-hydroxy fatty acid [OAHFA]), together with their simplified structures. Orange and red indicate the fatty alcohol (FAl) and ω-OH fatty acid (FA) moieties, respectively. (B) Schematic diagram of Cyp4f39+/+ (wild type), Cyp4f39–/– (Cyp4f39 whole-body knockout), and Tg-Cyp4f39–/– mice. Blue represents Cyp4f39 expression and gray indicates Cyp4f39 deficiency. (C) Body weight of Tg-Cyp4f39+/+ (n = 3), Tg-Cyp4f39+/– (n = 8), and Tg-Cyp4f39–/– (n = 7) mice at 10–12 months of age. Values presented are means ± SD. (D) Total lysates (20 μg) prepared from the heart, lung, liver, stomach, small intestine, kidney, epidermis, meibomian gland, and cornea of 12-month-old Tg-Cyp4f39–/– mice were separated by SDS-PAGE and subjected to immunoblotting with anti-FLAG and anti-GAPDH antibodies (loading control). (E, F) Photographs of 12-month-old Tg-Cyp4f39+/+ and Tg-Cyp4f39–/– mice. The photographs in (F) are enlargements of the areas enclosed by the yellow frames in panel (E). Tg +/+, Tg-Cyp4f39+/+; Tg–/–, Tg-Cyp4f39–/–; M. gland, Meibomian gland.