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. 2020 Apr 7;9:e53582. doi: 10.7554/eLife.53582

Figure 3. Normal formation of meibomian glands and cornea and normal gene expression in Cyp4f39-deficient mice.

Figure 3.

(A, B) Paraffin sections of 12-month-old Tg-Cyp4f39+/+ and Tg-Cyp4f39–/– mice were stained with hematoxylin and eosin. Bright-field images of meibomian glands (A, left panels) and cornea (B) photographed under a light microscope, and schematic diagrams of the meibomian gland acini (A, right panels) are presented. Scale bar, 25 μm. (C) Total RNAs were prepared from the meibomian glands of 12-month-old Tg-Cyp4f39+/+ (n = 3) and Tg-Cyp4f39–/– (n = 3) mice and subjected to real-time quantitative RT-PCR using specific primers for Awat1, Awat2, Far1, Far2, Soat1, Cyp4f39, or the housekeeping gene Hprt. Values are amounts of each mRNA relative to that of Hprt and represent means ± SD (*, p<0.05; Student’s t-test). Tg +/+, Tg-Cyp4f39+/+; Tg –/–, Tg-Cyp4f39–/–; MG, meibomian gland; BCL, basal cell layer; Ep, epithelium; St, stroma; En, endothelium.