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. 2020 Apr 7;9:e53582. doi: 10.7554/eLife.53582

Figure 6. Absence of type 1ω WdiEs and Chl-OAHFAs in meibomian glands from Cyp4f39-deficient mice.

Figure 6.

(A–C) Meibum lipids were prepared from 12-month-old Tg-Cyp4f39+/+ mice and subjected to product ion scanning by selecting the [C66:3 WdiE + H]+ ion, with an m/z value of 981.7, as the precursor ion. LC chromatogram of C66:3 WdiEs (A) and MS spectrum of type 1ω C66:3 WdiE (B) are shown. The structure of the type 1ω C66:3 WdiE is illustrated (C), as predicted from the fragment ion analysis in panel (B). (D, E) Lipids were extracted from the meibomian glands of 12-month-old control (Tg-Cyp4f39+/+ [n = 2] and Tg-Cyp4f39+/– [n = 1]) and Tg-Cyp4f39–/– (n = 3) mice. Then, type 1ω WdiEs (D) and Chl-OAHFAs (E) were analyzed by LC-MS/MS. The peak areas (left-hand panels) of lipid species with different chain lengths, with or without saturation, and their total amounts (right-hand panels) are shown. Values presented are means ± SD (*, p<0.05; **, p<0.01; Student’s t-test). The simplified structure of each lipid is shown below the graphs. nd, not detected; Tg –/–, Tg-Cyp4f39–/–.