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. 2020 Apr 7;9:e53582. doi: 10.7554/eLife.53582

Figure 7. Increased CEs and WEs in Cyp4f39-deficient mice.

Figure 7.

(A) Meibum lipids prepared from 12-month-old control (Tg-Cyp4f39+/+) and Tg-Cyp4f39–/– mice were separated by TLC using solution 1 (hexane/diethyl ether/acetic acid [90:25:1, v/v; left]) or solution 2 (hexane/toluene [1:1, v/v; right]) as the resolving solution, followed by detection using copper phosphate reagent. Asterisks indicate unknown lipids. (B) Lipids were extracted from the meibomian glands of 12-month-old control (Tg-Cyp4f39+/+ [n = 2] and Tg-Cyp4f39+/– [n = 1]) and Tg-Cyp4f39–/– (n = 3) mice, and CEs were analyzed by LC-MS/MS. The peak areas for CE species with different FA chain lengths, with or without saturation, and their total amounts (inset) are shown. Values presented are means ± SD (*, p<0.05; **, p<0.01; Student’s t-test). The simplified structure of CE is shown below the graph. Chl, cholesterol; TG, triglyceride; PL, phospholipid; Tg –/–, Tg-Cyp4f39–/–.