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. 2020 Apr 3;13:2843–2854. doi: 10.2147/OTT.S234250

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© 2020 Ge et al.

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ZGRF1 promoted cancer stemness: (A) Real-time PCR analysis revealed interference efficiency of the siRNA against ZGRF1 at 48 h after transfection was 85% and 75%, respectively. (B, C) Flow cytometry analyses of the ratios with CD44+/CD24− cells in the indicated cells with or without ZGRF1 knockdown were performed. (D, E) Tumor spheres of representative images of the indicated cells with or without ZGRF1 Knockdown; Scale bar = 400 μm. Histograms showed the mean numbers and diameters of spheres cultured. (F, G) Colony formation assay (n = 3). **P < 0.01, ***P < 0.001, and ****P < 0.0001 and error bars represent standard deviation from three times of independent experiments.