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. 2020 Apr 3;14:1351–1362. doi: 10.2147/DDDT.S223742

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© 2020 Zheng et al.

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DFE preconditioning inhibited I/R-induced cell apoptosis. (A) The numbers of apoptotic cells in the kidney of four groups were detected using TUNEL assay. (B) The expression levels of cell apoptosis-related proteins, including Bax, caspase 3, cleaved-caspase 3, caspase 9, cleaved-caspase 9 and Bcl-2, were measured using Western blot. (C) The expression levels of cleaved-caspase 3 in four groups were assessed using IHC. Data were shown as mean ± SD. **P < 0.01 vs sham group, ^#P< 0.05, ^##P < 0.01 vs I/R group.