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. 2020 Apr 3;13:2873–2886. doi: 10.2147/OTT.S243533

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© 2020 Li et al.

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miR-1253 was sponged by hsa_circ_0087862. (A) TargetScan and miRanda illustrated that miR-766-5p, miR-1253, miR-223-3p and miR-3139 possessed binding site for hsa_circ_0087862. (B) Luciferase reporter gene assay was performed to verify the targeting relationship between miRNAs and hsa_circ_0087862. (C) RIP assay was conducted using the Ago2 and IgG antibody to immunoprecipitate. The expression of hsa_circ_0087862 was detected by qRT-PCR. (D) miR-3139 expression in cells was detected by qRT-PCR. (E) miR-3139 expression in NSCLC tissues and normal tissues was assessed by qRT-PCR. The expression correlation between miR-1253 and hsa_circ_0087862 in NSCLC tissues was determined by Pearson’s correlation analysis. *P < 0.05. **P < 0.01. ***P < 0.001.