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. 2019 Nov 26;150(4):747–755. doi: 10.1093/jn/nxz277

FIGURE 7.

FIGURE 7

An oral gavage of ethanol in Sprague-Dawley rats did not change protein expression of SGLT1. (A) Western blot analysis of SGLT1 protein expression in whole cell homogenates from control and ethanol-treated Sprague-Dawley rats. The blots show a representative sample used for densitometric quantification. Separated control and ethanol samples were run on the same membrane but were not located next to each other. (B) Densitometric quantification of SGLT1 protein expression was not significantly changed in the whole cell homogenate fraction after 1 h exposure to ethanol (n = 4). (C) Western blot analysis of SGLT1 protein expression in the BBM from control and ethanol-treated Sprague-Dawley rats. The blots show a representative sample used for densitometric quantification. (D) Densitometric quantification of SGLT1 protein expression was not significantly altered in the BBM fraction after 1 h exposure of ethanol (n = 4). Values represent the means ± SEMs. Blots were normalized with an anti-ezrin antibody to assure equivalence of loading. All control and ethanol-treated samples were run on the same membrane. BBM, brush border membrane; SGLT1, SLC5A1.