TABLE 1.
Effect of ethanol on the kinetic parameters of Na-dependent glucose co-transport in rat intestinal epithelial cells in vitro and in vivo1
| Vmax, nmol/(mg protein·30 s) | Km, mM | |
|---|---|---|
| IEC-18 cells | ||
| Control | 4.9 ± 0.2 | 5.6 ± 0.5 |
| Ethanol | 5.1 ± 0.3 | 14.0 ± 1.0* |
| Sprague-Dawley rats | ||
| Control | 5.4 ± 0.6 | 12.4 ± 0.3 |
| Ethanol | 5.3 ± 0.5 | 15.3 ± 0.4* |
1
Na-dependent glucose uptake was determined by varying concentrations of glucose with phlorizin-sensitive 3H-OMG uptakes in the presence and absence of moderate ethanol for 1 h. Uptake for all concentrations was conducted at 30 s. Values represent the means ± SEMs. IEC, intestinal epithelial cell; OMG, O-methyl-d-glucose.
*
Denotes difference from control at that time and a significance of P < 0.01.