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. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: Reprod Toxicol. 2020 Feb 1;93:118–130. doi: 10.1016/j.reprotox.2020.01.009

Figure. 1.

Figure. 1.

Procedures of ovarian follicle vitrification and warming. Freshly harvested follicles were sequentially equilibrated in EG+PXZ and EDS vitrification solutions containing increasing concentrations of CPAs. Follicles were next loaded into 0.25 cc straw, cooled in LN2 vapor, and plunged into LN2 for long-term storage. The follicle retrieval was performed using a two-phase warming procedure followed by five sequential incubations in warming solutions with decreasing concentrations of sucrose. CPA, cryoprotective agents; PXZ, polyvinylpyrrolidone (PVP) K-12, Supercool X-1000™, and Supercool Z-1000™; LN2, liquid nitrogen.