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. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: Reprod Toxicol. 2020 Feb 1;93:118–130. doi: 10.1016/j.reprotox.2020.01.009

Figure 4.

Figure 4.

Effect of vitrification on the mRNA expression of ovarian steroidogenesis related genes and 17β-estradiol (E2) secretion. (A) Classic ovarian steroidogenesis pathway in follicular cells. (B) Concentration of E2 in follicle culture media on day 8 of eIVFG measured by ELISA. (C) mRNA expression levels of steroidogenesis related genes in freshly harvested and vitrified follicles on day 8 of eIVFG. Error bar: standard deviation. For E2 concentration analysis, N=6-7 follicles in each group for each replicate and three replicates was performed. For mRNA expression analysis, N=20-30 follicles in each group for each replicate and three replicates were included.