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. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: Reprod Toxicol. 2020 Feb 1;93:118–130. doi: 10.1016/j.reprotox.2020.01.009

Figure 7.

Figure 7.

Effect of different congeners of microcystins (MCs) on follicle growth, survival, and oocyte maturation during encapsulated in vitro follicle growth (eIVFG) using vitrified and warmed follicles. Isolated multilayered secondary follicles were vitrified using EDS based vitrification protocol. (A) Follicle diameter, survival rate, and MII oocyte percentage after in vitro oocyte maturation upon different congeners and concentrations of MCs exposure. (B) Representative images of follicles treated with different concentrations of MC-LF on days 0, 2, 4, 6, and 8 during elVFG. Error bar: standard deviation; *p< 0.05 and **p<0.01. N=8-10 follicles in each group for each replicate and 3 replicates were performed.