Table 1.
Recipes of vitrification and warming solutions.
| Vitrification solutions (EG+PXZ) | ||||
|---|---|---|---|---|
| Solution 1 | Solution 2 | Solution 3 | Solution 4 | |
| Total CPA concentration | 5% | 15% | 30% | 50% |
| CPA composition | 5% G | 7.5% G + 7.5% EG | 15% G + 15% EG | 25% G +25% EG |
| PXZ polymers | NA | NA | NA | 3% |
| Base medium | L-15 supplemented with 15% FBS and 1 μM L-Ascorbic acid 2-phosphate sesquimagnesium salt hydrate (AA2P) | |||
| Vitrification solutions (EDS) | |||
|---|---|---|---|
| Solution 1 | Solution 2 | Solution 3 | |
| Total CPA concentration | 0% | 15% | 40% |
| CPA composition | NA | 7.5% EG + 7.5% DMSO | 20% EG + 20% DMSO + 0.5 M sucrose |
| Base medium | L-15 supplemented with 20% FBS | ||
| Warming solutions (EG+PXZ and EDS) | |||||
|---|---|---|---|---|---|
| Solution 1 | Solution 2 | Solution 3 | Solution 4 | Solution 5 | |
| Sucrose concentration | 1 M | 0.75 M | 0.5 M | 0.25 M | 0 M |
| Base medium | L-15 supplemented 15% FBS and 1 μM AA2P | ||||
*
Notes: G: glycerol; EG: ethylene glycol; P: polyvinylpyrrolidone (PVP); X: Supercool X-1000™; Z: Supercool Z-1000™; DMSO: dimethyl sulfoxide; M: mol/L; NA: not applicable.