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. 2020 Apr 7;10:6023. doi: 10.1038/s41598-020-63093-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Vessel lumen diameter and cell wall thickness measurements at a given height using the Euclidean distance transform algorithm (EDT). Lumen (a), and cell wall (d), tomogram slices were preprocessed and binarised. The lumen diameter was measured by acquiring the distance of the centre pixel to all the border pixels (b), and different radius measurements are shown in (c), together with the frequencies. The cell wall thickness (e) is obtained by measuring the distance of the medial axis pixels to the border pixels with all the radius measurements represented in (f) by their frequencies.