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. 2020 Apr 7;10:6023. doi: 10.1038/s41598-020-63093-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Vessel implosion resistance analysis. (a) rendering indicating the structures selected for the analysis, the double wall thickness, t, and the lumen diameter, b, measured by the EDT algorithm to calculate the implosion resistance along the cellular height. The vessel cells of wild type (b), and C4H mutant plants (c) were individualised (rendering on the left) and the implosion resistance parameter, (t/b)², was calculated and plotted according to the cellular heights (right), for each cell (1 to 3). The red dotted line represents the average (t/b)² showing the displacement to lower values from wild type to the mutant cells.