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. 2020 Apr 7;10:6007. doi: 10.1038/s41598-020-62898-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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ULM images obtained from one individual flow channel data. (a) ULM intensity and velocity images of the flow channel obtained without MB separation. (b) The flow speed profile obtained without MB separation. (c) ULM intensity and velocity images of the same flow channel data obtained with MB separation method. (d) Well-developed parabolic flow speed profile obtained with the MB separation method. For one individual flow channel data, different flow velocities inherently exist along the radial direction. A stack of 2000 frames was used to produce the ULM image for this figure. The proposed method provided a higher number of localized MBs, permitting a denser population of the lumen of the flow channel.