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. 2020 Apr 7;10:6007. doi: 10.1038/s41598-020-62898-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Influence of data acquisition time. (a) Super-resolved microbubble localization images of the CAM surface vasculature with incrementally increasing data acquisition lengths (accumulation time). Most of the microvessels, down to the ~10 µm diameter range, could be resolved within ~4 seconds using the MB separation technique. (b) The saturation rate of the vessel region indicated by the rectangle in Fig. 5a demonstrates that the vessel lumen was mostly populated within the first 2–3 seconds and plateaued beyond 4 s for the MB separation method, while for conventional ULM the saturation curve shows that the time to reach the plateau was much longer (>8 s).