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. 2020 Apr 7;10:6007. doi: 10.1038/s41598-020-62898-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Application of proposed method to chicken embryo tumor and brain. ULM images were generated for CAM tumor both (a) without microbubble separation and (b) with microbubble separation. The corresponding images depict ULM velocity maps (c) without microbubble separation and (d) with microbubble separation. Likewise, ULM images of chicken embryo brains were generated (e) without microbubble separation and (f) with microbubble separation. As with the tumor data, the velocity maps (g) without microbubble separation were less populated than the velocity maps (h) with microbubble separation.