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. 2020 Apr 7;10:6032. doi: 10.1038/s41598-020-63034-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of SNP of GSTP1 A313G and its 3D monomer structure. (A) A complete gel picture of the PCR-product of GSTP1 from six samples; M-marker. (B) A complete gel picture shows the result of PCR-RFLP analysis for GSTP1 SNP. The presence of Alw26I restriction site yielded 328 and 105 bp fragments for the A allele and 222, 105 and 106 bp fragments for the G allele. (C) Partial sequence chromatograms of GSTP1 A313G polymorphism (arrow marked) are shown from three subjects whose RFLP data are depicted in B. (D) 3D structure of wild GSTP1 I105 (Camel colour) and mutant GSTP1 V105 (Carolina blue colour). Altered sites were marked by arrows and showed the amino acids and its position.