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. 2020 Apr 7;10:6028. doi: 10.1038/s41598-020-63234-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of functionalized multifunctional nanoparticles: (a) In vitro MR. Comparison of longitudinal relaxation time distributions of water (-■-), empty PEG-cHANPs suspension (-●-), PEG-cHANPs loaded with Gd-DTPA (-▲-); PEG-cHANPs co-loaded with ATTO488 and Gd-DTPA (-◀-); (b) Optical Imaging of co-loaded PEG-cHANPs under Confocal Microscopy (acquisition spectra 500–530 nm), scale bar 10 μm; (c) In vitro cytotoxicity: FSC signal of co-loaded-PEG-cHANPs in contact with U87 MG cells in three different serum condition at three different time points. Abbreviation: FSC, forward scattering.