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. 2020 Apr 7;10:5992. doi: 10.1038/s41598-020-62856-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Honeydew tinted area from DCEF27-, DCEF28-, and DCEF32- silenced ACPs after feeding with ‘Rangpur lime’ leaves. (B) Mortality rates of D. citri on Rangpur lime leaves after treatment with dsRNAs for the DCEF27, DCE28, or DCEF32 effectors. ACPs were treated with effector dsRNAs in vitro and then transferred to the citrus plants for feeding. The mortality rates were evaluated daily during a 120-hour period. The data correspond to the average of four independent experiments. Different letters correspond to statistically significant differences amongst the treatments (Tukey test, P-value < 0.05).