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. 2020 Apr 7;10:5992. doi: 10.1038/s41598-020-62856-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Relative gene expression of the candidate effectors DCEF26 (A), DCEF27 (B), DCEF28 (C), DCEF32 (D), DCEF33 (E) and DCEF35 (F) compared with the GFP control. Gene expression evaluation occurred on ACP adults after feeding with an artificial diet containing dsRNAs for the effectors or dsRNAs for GFP for five days. The data correspond to the mean of three independent experiments. *corresponds to statistically significant differences amongst the treatments (t-test, P-value < 0.05).