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. 2020 Apr 7;11:1729. doi: 10.1038/s41467-020-15537-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a A diagram showing bilateral injection of AAV2-hSyn-DIO-mCherry, AAV2-hSyn-DIO-hM3D(Gq)-mCherry, or AAV2-hSyn-DIO-hM4D(Gi)-mCherry into the PVH of Ntrk2^CreER/+ mice. b A confocal imaging showing mCherry expression of injected AAV in the PVH. Brain sections were counter-stained with DAPI. The scale bar represents 100 μm. c Inhibition of PVH^TrkB neurons stimulated food intake during the light cycle. n = 5 and six mice for AAV2-hSyn-DIO-mCherry (mCherry) and AAV2-hSyn-DIO-hM4D(Gi)-mCherry [hM4D(Gi)] groups, respectively. Two-way ANOVA with post hoc Tukey’s multiple comparisons; F_{(3, 72)} = 35.23, P < 0.0001 for virus and treatment; ****p < 0.0001 when compared to the hM4D(Gi)-vehicle (Veh) group. d Inhibition of PVH^TrkB neurons stimulated food intake during the dark cycle. n = 6 and six mice for mCherry and hM4D(Gi) groups, respectively. Two-way ANOVA with post hoc Tukey’s multiple comparisons; F_{(3, 76)} = 31.49, P < 0.0001 for virus and treatment; ****p < 0.0001 when compared to the hM4D(Gi)-Veh group. e Activation of PVH^TrkB neurons suppressed food intake during the dark cycle. n = 6 and seven mice for AAV2-hSyn-DIO-mCherry (mCherry) and AAV2-hSyn-DIO-hM3D(Gq)-mCherry [hM3D(Gq)] groups, respectively. Two-way ANOVA with post hoc Bonferroni’s multiple comparisons; F_{(3, 22)} = 17.27, P < 0.0001 for virus and treatment; ****P < 0.0001 when compared to the hM3D(Gq)-Veh group. f Activation of PVH^TrkB neurons suppressed fasting-induced appetite. n = 6 and seven mice for mCherry and hM3D(Gq) groups, respectively. Two-way ANOVA with post hoc Bonferroni’s multiple comparisons; F_{(3, 22)} = 10.47, P < 0.0001 for virus and treatment; *P < 0.05, **P < 0.01, and ****P < 0.0001 when compared to the hM3D(Gq)-Veh group. Error bars indicate SEM. Source data are provided as a Source Data file.