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. 2020 Apr 7;11:1729. doi: 10.1038/s41467-020-15537-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — a–c Refeeding after overnight food deprivation induced Fos expression in PVH^TrkB neurons projecting to the VMH. PVH^TrkB→VMH neurons were labeled by tdTomato and CTB488 injected into the VMH in tamoxifen-treated Ntrk2^CreER/+;Rosa26^Ai9/+ mice (a). Arrowheads denote neurons positive for tdTomato and CTB488, while arrows indicate neurons positive for tdTomato, CTB488 and Fos (b). Fos induction in PVH^TrkB→VMH neurons under fasting and refeeding conditions was quantified (c). n = 3 mice in the fasted group and three mice in the refed group. Two-tailed unpaired t test; ***P = 0.0004. d–f Refeeding after overnight food deprivation induced Fos expression in PVH^TrkB neurons projecting to the LPBN. PVH^TrkB→LPBN neurons were labeled by tdTomato and CTB488 injected into the LPBN in tamoxifen-treated Ntrk2^CreER/+;Rosa26^Ai9/+ mice (d). Arrowheads denote neurons positive for tdTomato and CTB488, while arrows indicate neurons positive for tdTomato, CTB488 and Fos (e). Fos induction in PVH^TrkB→LPBN neurons under fasting and refeeding conditions was quantified (f). n = 3 mice in the fasted group and three mice in the refed group. Two-tailed unpaired t test; **P = 0.006. Error bars indicate SEM. Scale bars represent 50 μm. Source data are provided as a Source Data file.