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. 2020 Apr 7;11:1729. doi: 10.1038/s41467-020-15537-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 9 — a PVH^TrkB→LPBN neurons were labeled with injection of AAV2-Ef1a-fDIO-mCherry into the PVH and AAV2retro-Ef1a-DIO-FLPo into the LPBN of Ntrk2^CreER/+ mice. b, c Patch-clamp recording of an mCherry-labeled PVH^TrkB→LPBN neuron. Scale bars represent 25 μm. d A representative trace showing neuronal firing before and after bath application of BDNF. e Quantification of baseline neuronal firing (Base) and neuronal firing 40 min after bath application of BDNF (BDNF). Two-tailed paired Student’s t test; *P = 0.019. Source data are provided as a Source Data file.