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. 2020 Apr 7;11:1727. doi: 10.1038/s41467-020-15455-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Sequence alignment of loops 1 and 2 of FabF with representative KS sequences from non-iterative type II PKS (Iga11, ApeO), iterative type II PKS (act-KS), type I FAS (Human-FAS-KS), and type I PKS (DEBS KSs). The putative gating residue (Phe400 in FabF) for each synthase is outlined in a black box. b Single time point crosslinking gels of 1c-AcpP (C2αBr-AcpP) and 1a-AcpP (C12αBr-AcpP) with FabF gating mutants. c Single time point crosslinking gels of 2b-AcpP (C3Cl-AcpP) and 2a-AcpP (C8Cl-AcpP) with FabF gating mutants. d Densitometric analysis of single time-point crosslinking efficiency of 1c-AcpP (C2αBr-AcpP) and 1a-AcpP (C12αBr-AcpP) with FabF mutants. e Densitometric analysis of single time-point crosslinking efficiency of 2b-AcpP (C3Cl-AcpP) and 2a-AcpP (C8Cl-AcpP) with FabF mutants. All crosslinking experiments from b and c were performed as biologically independent experiments (n = 3) and all data represented in d and e are the average crosslinking efficiency of each mutant normalized to the average of wt FabF crosslinking efficiency. The error bars in d and e are represented as standard deviation (±SD) and the individual normalized measurements from each independent experiment are overlaid on top of the associated bar plot as open circles. Source data for all experiments are provided as a Source Data file.