Skip to main content
. 2020 Apr 7;10:5995. doi: 10.1038/s41598-020-62879-y

Figure 2.

Figure 2

Characterization of large deletions between exon 5 and 6. Panel a) PCR scheme with primers situated in 5′ of exon 5 and in 3′ of exon 6 revealing a band of 3267 bp for a WT or a theoretical 357 bp band in case of double DNA cut and deletion without indels. The location of the two sgRNA used are indicated (red bars). Panel b) For 5 lambs with deletions, the sequence of these PCRs showed variable deletions with insertion (#61, #63 and #78) or not (#61 and #66) depending on microhomologies. The sequences recognized by the two sgRNA used are indicated (red letters) and the PAMs in black bold. Between the sequences the size of the deletions and of the insertions and at the right hand side the real PCR size for each lamb with the final outcomes. PTC: Premature Termination Codon.