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. 2020 Apr 7;10:5995. doi: 10.1038/s41598-020-62879-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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oOTOF gene knock-out/knock-in exon 5 and 6. Panel a) Example of typical result for exon 5 PCR (left lines) and Spe I exon 5 PCR digest (right lines). Main band is around 500 bp. The bands above the main band in all animals except #73 (WT/WT) are heteroduplexes which means that NHEJ and/or KI occurred. Spe I digest revealed two bands (asterisk) under the main band of animals #74, #75 and #97 and means that a KI occurr. Panel b) Example of typical result for exon 6 PCR. Main band is around 500 bp. The bands above the main band in all animaedls except #74 and #76 (WT/WT) are heteroduplexes which means NHEJ occurred. Animal #75 shows a smaller band of ~400 bp under the main band which identifies a 198 bp deletion.