Figure 1.

The effect of Mβ-CD on ouabain-sensitive Na/K-ATPase ion-transport activity (⁸⁶Rb⁺ uptake) and characteristics in different cell types: Before initiation of ⁸⁶Rb⁺ uptake, cells were pretreated with 10 mM of Mβ-CD for 30 min at 37 °C. The ⁸⁶Rb⁺ uptake was calibrated with protein content. Data were expressed as the percentage of control ouabain-sensitive ⁸⁶Rb⁺ uptake (Mean ± SD). n = 8–12 independent experiments (each performed in triplicate) for each cell type. (a) LLC-PK1, n = 9. (b) C2-9, n = 8. (c) SYF + c-Src, n = 12. (d) SYF, n = 8. **p < 0.001 vs. control (CTL). Comparison of cell surface Na/K-ATPase α1 subunit by cell surface biotinylation (e) and protein carbonylation (f) between LLC-PK1 and C2-9 cells, and between SYF and SYF + c-Src cells. Data were presented as Mean±SD, n = 3. **p < 0.05 vs. LLC-PK1 or SYF + c-Src cells.