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. 2020 Mar 25;23(4):101005. doi: 10.1016/j.isci.2020.101005

Figure 1.

Figure 1

Cell Viability Studies of Ber-D and Berberine

(A) Concentration-dependent (0.5, 1, 2.5, 5, 10, 25, 35, 50, and 100 μM) and comparative cell viability of Ber-D and berberine in PC12 cells.

(B) Quantification of Rho123 fluorescence at 534 nm (λex = 511 nm) corresponding to MMP of PC12, HeLa, and HEK cells treated independently with Ber-D (50 μM) and berberine (50 μM).

(C) Fluorescence microscopic images of Ber-D (50 mM) or berberine (50 mM) treated HeLa cells using Mitotracker green FM (mitochondria-specific dye) to study the mitochondrial morphology. Scale bar, 50 μM. Each experiment was repeated (n = 16, for cell viability; n = 4, for MMP quantification); error bars represent the standard deviation (SD). Ber, berberine.