Figure 2.

Metal Chelation and Anti-aggregation Properties of Ber-D

(A) Absorbance spectra of Ber-D, Ber-D+Cu^II, and Aβ42+Cu^II+Ber-D complex. Inset: proposed structure for Ber-D and two His units of Aβ chelation with Cu^II to form the Aβ42+Cu^II+Ber-D complex.

(B) Aβ42 (10 μM) was incubated with Ber-D (10, 20, 50, and 100 μM) or berberine (100 μM) in a concentration-dependent manner, and aggregation inhibition was quantified through fluorescence measurement of ThT at 485 nm (λ_ex = 450 nm) after 48 h.

(C) Dot blot analysis of Aβ42 aggregates to study the anti-aggregation properties of Ber-D and berberine on the formation of Aβ42 fibrillar aggregates and Aβ42 oligomeric species. OC and A11 antibody were used for specific detection of Aβ42 fibrillar aggregates and Aβ42 oligomeric species, respectively.

(D) Aβ42 (10 μM) and Cu^II were incubated alone and independently with Ber-D (10, 20, 50, and 100 μM) or berberine (100 μM) in a concentration-dependent manner, and aggregation inhibition was quantified through fluorescence measurement of ThT at 485 nm (λ_ex = 450 nm) after 24 h. Each experiment was repeated thrice (n = 3); error bars represent the standard deviation (SD) of the NFI. Ber, berberine.

See also Figures S1 and S4.