Skip to main content
. 2020 Mar 25;23(4):101005. doi: 10.1016/j.isci.2020.101005

Figure 5.

Figure 5

Mechanism of Aβ Toxicity in PC12 Cells

(A) PC12 cell viability observed after independently incubated (24 h) with Aβ42 (10 μM) and Ber-D or berberine (Ber).

(B) Cell viability of PC12 cells after independently incubated (24 h) with Aβ42 (10 μM), CuII (10 μM), ascorbate (200 μm), and Ber-D or berberine.

(C) Fluorescence microscopic images of Rho123-treated PC12 cells after incubation (12 h) with Aβ42 (10 μM) and Ber-D or berberine (20 μM). Scale bar, 50 μM.

(D) Western blot analysis of the protein levels of cyt C in PC12 cells treated with Aβ42 (10 μM) and Ber-D or berberine (20 μM). Statistical analysis for the effect of Ber-D or berberine on the expression of Cyt C obtained from the western blot.

(E) Quantification of caspase 3 generations in PC12 cells treated with Aβ42 (10 μM) and Ber-D or berberine (20 μM). Ber, berberine.

Statistical analysis was performed through ordinary one-way ANOVA or Kruskal-Wallis one-way ANOVA test: ∗∗, p value 0.005; ∗, p value 0.05.