Table 3.

Primers used to analyze insert in presumed PSY1 mutants.

Primer	Sequence (5′ 3′)	Comments
PSY_F	TGCGGCCTCAATCCAATGTTTC
PSY+400F	CGTGAACCATCACCCTAATCAAG
PSY_2R	GTCCACCAGCTCGTCAGTTCGCCGG	317-bp amplicon in WT. 3′ end (9 bp) matches insert in Colony 15, gives ~480 bp amplicon with PSY_F
PSY+500R	CCAATCAGGGTCCAGGGAAC	Pairs with PSY_F forward primer
PSY+1000R	TATTGCTTCCTCTGCTGGTTCG	Pairs with PSY_F or PSY+400F forward primer
PSY+1500R	TGCAAGTCAAATCTGCAAGCAC	No priming in Colony 15 with PSY_F or PSY+400F