Table 1.
Gene | Nucleotide sequence |
---|---|
Mybbp1a | Probe: 5’-VIC-CGCCTAATCACTGGGCTTGG-BHQ2–3’ Forward primer: 5’-TCGGAGATGAAATATGCC-3’ Reverse primer: 5’-ATGGGATGTCTTCAAAAGA-3’ |
NM_016776.2a | |
Ppargc1a | Probe: 5’-VIC-TAACTCCTCCCACAACTCCTCCTC-BHQ2–3’ Forward primer: 5’-CGAACCTTAAGTGTGGAA-3’ Reverse primer: 5’-AGCCTTGAAAGGGTTATC-3’ |
NM_008904.2 | |
Prkn | Probe: 5’-VIC-CACGATGCTCAACTTGGCTACTC-3’ Forward primer: 5’-GTCACAAGACTCAACGATC-3’ Reverse primer: 5’-TGCTCTTCTCCAAGGATC-3’ |
NM_016694.3 | |
Nrf1 | Probe: 5’-VIC-AGCATGATCCTGGAAGACCTCG-BHQ2–3’ Forward primer: 5’-CAGCACCTTTGGAGAATG-3’ Reverse primer: 5’-GGCAGCTCTGAATTAACC-3’ |
NM_001164226.1 | |
Zfp746 | Probe: 5’-VIC-ACTGGTCTCCCTGGACTATGCC-BHQ2–3’ Forward primer: 5’-GAGCTCTACAAGCATGTG-3’ Reverse primer: 5’-TTGCCTTGTTCAATCTGG-3’ |
NM_001163475.1 | |
Kif1b | Probe: 5’-VIC-CAAGGAGTCCAAGTGCATCATTCAG-BHQ2–3’ Forward primer: 5’-CCTTCAATTCTCGAGAGA-3’ Reverse primer: 5’-CCAGTAGGAGTAGTCAAA-3’ |
NM_207682.2 | |
Bcat2 NM_001243053.1 | Probe: 5’-FAM-CGGATACACTCCAACAGCTCCTGCTTGT-BHQ1–3’ Forward primer: 5’-TCAACATGGACAGGATGCTACG-3’ Reverse primer: 5’-CCAGTCTTTGTCTACTTCAATGAGC-3’ |
Psmd7 NM_010817.2 | Probe: 5’-FAM-AGTCCTAGGTCCTTTGGCTTCACGTCGA-BHQ1–3’ Forward primer: 5’-CTGCACAAGAATGATATCGCCATC-3’ Reverse primer: 5’-CTCCACTGAGATGTAGGCTTCG-3’ |
FAM and VIC – fluorescent dyes; BHQ1 and BHQ2 – fluorescence quenchers.
Numbers in the database GenBank (Accession numbers).