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. 2020 Mar 5;36:100967. doi: 10.1016/j.molmet.2020.02.010

Figure 2.

Figure 2

A combination of six miRNAs rescues myotube morphology and reverses induction of the focal adhesion gene cluster following DGCR8 deletion. Dgcr8 KO and control myoblasts were transfected with control mimics, the indicated individual six miRNA mimics or their combination (6× miRNA) two days after beginning of the tamoxifen incubation. Twenty-four hours after transfection, myoblast differentiation was induced for 48 h. Myotube morphology was analyzed using immunofluorescence for desmin (A) and brightfield microscopy (B), 10× magnification. Scale bar = 50 μm. C. KEGG pathway analysis of RNA isolated from control, Dgcr8 myotubes and Dgcr8 myotubes transfected with the combination of six miRNAs. D. RNA levels as determined by RNA deep sequencing in control and DGCR8 knockout cells with or without transfection of the six miRNA mimics, n = 3. Shown are all genes that are significantly upregulated in the knockout cells and significantly downregulated after transfection with the mimics (p value < 0.05 according to manufacturer's analysis).