Skip to main content
. 2020 Mar 13;17:9–20. doi: 10.1016/j.omto.2020.03.002

Figure 1.

Figure 1

Verification of the Binding of Aptamers to Their Target Proteins

Aptamers were modified with a 3′-terminal biotin modification kit, incubated with the target proteins in EP tube and analyzed by 8% or 10% native PAGE gel. (A and C) The binding of CD28Apt7 to CD28 protein (A) and Del60 to CTLA-4 protein (C). The more protein added, the more obvious the binding band was. BSA or TIM3 were used as the negative control target proteins. (B and D) The cold competitive binding results of the aptamers to CD28protein (B) and CTLA-4 protein (D), respectively. The biotin unlabeled aptamer was used to compete the biotin labeled aptamer to the same target protein. (E and F) The binding (E) and cold competitive binding (F) of the dimer of CD28Apt7 to CD28 proteins.