Figure 5.

X-polymer Nanoparticles Specifically Bind to Mouse T Cells and Tumor Cells

X-polymer nanoparticles (green fluorescence) were assembled with the FAM-labeled 3WJ-2Del60 sequence, and T cells were stained with anti-CD3 antibody (red fluorescence) labeled with APC. The co-localization of APC fluorescence and FAM fluorescence was observed under the confocal microscope (A). The binding of X-polymer nanoparticles to T cells was confirmed by flow cytometry as shown in (B). The Cy5-labeled 3WJ-2Del60 sequence was used to assemble X-polymer nanoparticles (red fluorescence). (C–E) The binding of X-polymer nanoparticles to B16 cells marked with CFSE (green fluorescence) (C), MC38 cells marked with Hoechst reagent (blue fluorescence) (D), and LO2 cells marked with CFSE (green fluorescence) (E) was observed under the confocal microscope. The ruler in the picture indicates a length of 20 μm.