Specifications table 

Subject	Biology
Specific subject area	Reproductive biology
Type of data	Table
	Figure
How data were acquired	Sequencing of RNA from rat granulosa cells
Data format	Raw (FASTQ) and analysed (Excel tables)
Parameters for data collection	RNA sequencing was performed on granulosa cells collected from the ovaries of gonadotropin treated wildtype and Esr2-null mutant rats.
Description of data collection	Four-week-old wildtype and Esr2-null Holtzman rats were treated with 30IU of PMSG. Forty-eight hours after PMSG administration, rats were injected with 30IU of hCG. Granulosa cells were collected from the rat ovaries before and after gonadotropin treatment, RNAs were purified and analysed by mRNA-sequencing. Gonadotropin-induced genes and the differentially expressed genes in Esr2-null granulosa cells were identified by analyses of the transcriptome data using CLC Genomics Workbench.
Data source location	University of Kansas Medical Center, Kansas City, KS 66160, USA
Data accessibility	Repository name: SRA
	Data identification number: PRJNA551764 and PRJNA551766
	Direct URL to data: https://www.ncbi.nlm.nih.gov/sra/ PRJNA551764 and https://www.ncbi.nlm.nih.gov/sra/ PRJNA551766